A dansyl-derivatized phytic acid analogue as a fluorescent substrate for phytases: experimental and computational approach

• Synthesis of a chromogenic analogue myo -inositol hexaphosphate. Amenable to g-scale. Docking experiments confirmed the compatibility with active site phytases. Phytases operate experimentally this fluorogenic substrate. A new pentakisphosphate was synthesized, which featured dansyl group at position C-5. The fluorescent tag removed from inositol by 6-atom spacer prevent detrimental steric interactions in catalytic PEG linker used order enhance hydrophilicity and biocompatibility artificial Computational studies showed favorable positioning Enzymatic assays demonstrated that tethered processed two recombinant phytases Phy-A Phy-C, classified respectively as acid alkaline phytases, similar rates phosphate release compared their natural